Mass Fingerprinting of Complex Mixtures: Protein Inference from High-Resolution Peptide Masses and Predicted Retention Times
نویسندگان
چکیده
منابع مشابه
Proteomic data mining using predicted peptide chromatographic retention times
Correct identification of proteins from peptide fragments is important for proteomic analyses. Peptides are initially separated by Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC) before Mass Spectrometry (MS) identification. At the present time, peptide fragment retention (separation) time is not used as a useful scoring filter for identification of the peptide fragments and the...
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Identification of proteins by mass spectrometry (MS) is an essential step in proteomic studies and is typically accomplished by either peptide mass fingerprinting (PMF) or amino acid sequencing of the peptide. Although sequence information from MS/MS analysis can be used to validate PMF-based protein identification, it may not be practical when analyzing a large number of proteins and when high...
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The high-resolution uv analyzer has been used to investigate the effect of diet on urine profile. In addition, new applications in the food and beverage industry appear promising. More than 100compounds, acidic to basic, can be separated. The clinical analyst may be interested in scanning a wide variety of compounds or in rapid separation of only a few compounds. For the latter purpose, the eff...
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ژورنال
عنوان ژورنال: Journal of Proteome Research
سال: 2013
ISSN: 1535-3893,1535-3907
DOI: 10.1021/pr400705q